Kan Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: 0.312 μg/ml.
Host	Sheep
Clonality	Polyclonal
Immunogen	Kan-BTG
Specificity	This antibody recognizes Kan.
Formulation	20mM Phosphate, 150mM Sodium Chloride State: Ig Fraction State: Liquid Ig fraction prepared by Caprylic Acid and Ammonium Sulphate precipitation procedures Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Kan (also known as Kan A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kan is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is Kan sulfate. Kan is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for Kan resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the Kan resistance gene are plated on Kan (50-100 ug/ml) containing agar plates or are grown in media containing Kan (50-100 ug/ml). Only the bacteria that have successfully taken up the Kan resistance gene become resistant and will grow under these conditions. As a powder Kan is white to off-white and is soluble in water (50 mg/ml). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria; it may be incapable of giving resistance to Kan to bacteria even if gene transfer occurs.
Synonyms	Kan A, Kantrex
Reference Data

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