p27 KIP 1 (CDKN1B) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	ELISA: 1/1,000. Western blotting: 1/50-1/100. Immunofluorescence: 1/10-1/50.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide selected from the C-terminal region of Human 27Kip1.
Specificity	This antibody recognizes Human 27Kip1. Other species not tested.
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein A column, followed by peptide affinity purification
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	22073 kDa
Gene Name	cyclin-dependent kinase inhibitor 1B
Database Link	Entrez Gene 1027 Human UniProt ID P46527
Background	p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Function: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. Cellular Location: Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export Tissue Location: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Synonyms	p27Kip1
Reference Data

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