TUG (ASPSCR1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/100~1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide selected from the C-terminal region of Human ASPSCR1.
Specificity	This antibody is specific to ASPSCR1/TUG (C-term).
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative. State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein A Chromatography followed by peptide affinity purification.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ASPSCR1, UBX domain containing tether for SLC2A4
Database Link	Entrez Gene 79058 Human UniProt ID Q9BZE9
Background	ASPSCR1 is tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. Insulin stimulates glucose uptake in fat and muscle by mobilizing the GLUT4 glucose transporter. GLUT4 is sequestered intracellularly in the absence of insulin, and is redistributed to the plasma membrane within minutes of insulin stimulation. Bogan et al. (2003) described a functional screen to identify proteins that modulate GLUT4 distribution, and identified TUG as a putative tether, containing a UBX domain, for GLUT4. They identified the ASPL protein as the probable human homolog of murine TUG. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4; in 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin. Distinct regions of TUG are required to bind GLUT4 and to retain GLUT4 intracellularly in transfected, nonadipose cells. Bogan et al. (2003) concluded that TUG traps endocytosed GLUT4 and tethers it intracellularly, and that insulin mobilizes this pool of retained GLUT4 by releasing this tether. Bogan et al. (2003) found that TUG exists in 2 isoforms, the longer of which contains a sequence at the amino terminus with similarity to ubiquitin that is predicted to be 550 residues long. The probable start codon in the short form is equivalent to methionine-78 of the long form.
Synonyms	ASPL, RCC17, TUG, UBXD9, UBXN9
Note	Calculated MW: 69,990 Da (Isoform 2)
Reference Data

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