uidA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized IgG fraction. Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Molar radio: Biotin/IgG~ 6.2
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P05804
Background	Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
Synonyms	GUSB, Beta-G1
Reference Data

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