ALD2 (pan ALDH) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques, to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/100,000.
Reactivity	Bakers Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Aldehyde dehydrogenase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore by adding 0.5 ml of sterile distilled water
Concentration	lot specific
Purification	Solid Phase Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Database Link	UniProt ID P47771
Background	Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. It Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).
Synonyms	ALD5, Aldehyde dehydrogenase [NAD(P)+] 1
Reference Data

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