DATF1 (DIDO1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of DIDO1 / Dio-1 protein. (region surrounding Ser186)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~244 kDa
Gene Name	death inducer-obliterator 1
Database Link	Entrez Gene 23856 MouseEntrez Gene 11083 Human UniProt ID Q9BTC0
Background	DIDO1 / Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.
Synonyms	Death-inducer obliterator 1, DATF1, C20orf158, KIAA0333
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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