IRS1 pSer612 Rabbit Polyclonal Antibody
CAT#: AP20954PU-N
IRS1 pSer612 rabbit polyclonal antibody, Aff - Purified
Need it in bulk or conjugated?
Get a free quote
CNY 4,437.00
货期*
2周
规格
Specifications
Product Data | |
Applications | IHC |
Recommend Dilution | Immunohistochemistry in paraffin sections: 1/50 - 1/200. |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Specificity | This antibody detects endogenous levels of IRS-1 protein. (region surrounding Ser612) |
Formulation | Phosphate buffered saline (PBS), pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide |
Concentration | 1.0 mg/ml |
Purification | Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, purity is > 95% (by SDS-PAGE) |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Predicted Protein Size | ~ 132,180 kDa |
Gene Name | insulin receptor substrate 1 |
Database Link | |
Background | Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. Tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while Serine (Ser)/Threonine (Thr) phosphorylation of IRS-1 can either enhance or negate insulin effects. Tyrosines 465, 612, 632, 662, 941 and 989 of IRS-1 resemble YXXM motifs that upon phosphorylation are predicted to bind SH2 domains in the p85 regulatory subunit of PI3K, resulting in activation of p110 catalytic subunit. SHP-2 binding to IRS-1 can occur upon phosphorylation at Tyr 1179 and Tyr 1229. GRB2 binding can occur upon phsophorylation at Tyr 896. Rodent Ser 99 and Thr 502 of IRS-1 are casein kinase II-dependent phosphorylation sites. There is an increase in Ser 636 phosphorylation of IRS-1 in primary skeletal muscle cells from patients with type 2 diabetes. IGF-I and anisomycin treatment converge downstream onto mTOR and PKC δ to induce IRS-1 Ser 312 phosphorylation. Insulin resistance in the aorta of hypertensive rats is associated with elevated IRS-1 phosphorylation at Ser 307 and increased SAPK/JNK activation. IRS-1 contains three putative binding sites for 14-3-3 protein at Ser 270, Ser 374 and Ser 641 that are capable of phosphorylation. |
Synonyms | Insulin receptor substrate 1, IRS-1 |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...