HXK1 Rabbit Polyclonal Antibody

CAT#: AP21320AF-N

HXK1 rabbit polyclonal antibody, Azide Free



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CNY 3,402.00


货期*
5周

规格
    • 10 mg

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommend Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Recommended Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
Reactivity Bakers Yeast
Host Rabbit
Clonality Polyclonal
Immunogen Hexokinase isolated and purified from Baker’s Yeast.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity Saccharomyces cerevisiae Hexokinase.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized Hyperimmune IgG fraction
Reconstitution Method Restore with 1.0 ml sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Background Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.
In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 molecules/cell in log phase SD medium.
Synonyms HXK1, HKA, Hexokinase PI, Hexokinase-A
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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