Luciferase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Reactivity	Photinus pyralis
Host	Rabbit
Clonality	Polyclonal
Immunogen	Luciferase isolated and purified from Photinus pyralis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml sterile distilled water.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08659
Background	Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

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