Proteinase K / PROK Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer's own choice. Recommended Working Dilutions: vary widely, but may be between 1/10000 and 1/1000000. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Proteinase K isolated and purified from Tritirachium album. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	IgG (7S) fraction of polyclonal Rabbit antiserum to Proteinase K from Tritirachium album. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized Hyperimmune purified IgG fraction
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P06873
Synonyms	Endopeptidase K, Tritirachium alkaline proteinase
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.