HNF 4 alpha (HNF4A) (2-15) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/32000. Immunohistochemistry on Paraffin Sections: 2 µg/ml. Western Blot: 0.1 - 0.3 µg/ml.
Reactivity	Canine, Human, Porcine, Bat, Equine, Hamster, Monkey, Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-terminus of human HNF4A / HNF4 (NP_849180.1; NP_000448.3; NP_849181.1)
Specificity	This antibody detects HNF4 alpha / TCF14 (N-term). This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Formulation	Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hepatocyte nuclear factor 4 alpha
Database Link	Entrez Gene 3172 Human UniProt ID P41235
Background	Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism. HNF4 alpha is essential for hepatocyte differentiation during liver development, metabolic regulation, and liver function. Although HNF4 alpha is a tissue-specific transcription factor, it is also an essential regulator of early embryonic events. Mutations in the HNF4 alpha gene are responsible for type-1 maturity-onset diabetes of the young (MODY1), which is characterized by a defect in insulin secretion. Mutations in HNF4 alpha binding sites have been linked to hemophilia B Leyden, atherosclerosis, hepatitis, and other diseases. HNF4 alpha has been linked to liver cancer via hepatitis and hepatitis B viral infections. Mouse embryos lacking HNF4 alpha fail to complete gastrulation due to dysfunction of the visceral endoderm. Recently, it has been shown that HNF4 alpha is critically involved in the PXR- and CAR-mediated transcriptional activation of CYP3A4, a gene involved in the metabolism of a wide range of drugs Tirona et al. 2003. At least four isoforms (HNF4 alpha1/HNF4B, HNF4 alpha2/HNF4A, HNF4 alpha3/HNF4C, and HNF4 alpha4) are produced by alternative splicing.
Synonyms	HNF4A, HNF4, NR2A1, Hepatocyte nuclear factor 4-alpha, Transcription factor HNF-4, Transcription factor 14
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways	Maturity onset diabetes of the young

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