p27 KIP 1 (CDKN1B) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect P27 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect P27 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunoprecipitation.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide mapping at the C-terminal end of P27 of human origin
Specificity	This antibodyd etects CDKN1B / KIP1 at C-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase inhibitor 1B
Database Link	Entrez Gene 12576 MouseEntrez Gene 83571 RatEntrez Gene 1027 Human UniProt ID P46527
Background	Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.
Synonyms	p27Kip1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer

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