TNFRSF1A (195-211) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 0.1-0.5 μg/ml. Immunohistochemistry on Paraffin Sections: 0.5-1 μg/ml. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Reactivity	Goat, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequencein the middle region of Human TNFR1 (aa 195-211).
Specificity	This antibody detects CD120a / TNFR1 (195-211). No cross reactivity with other proteins.
Formulation	0.9 mg NaCl, 0.2 mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction Stabilizer: 5 mg BSA Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor receptor superfamily member 1A
Database Link	Entrez Gene 21937 MouseEntrez Gene 25625 RatEntrez Gene 7132 Human UniProt ID P19438
Background	Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.
Synonyms	Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Cytokine-cytokine receptor interaction, MAPK signaling pathway

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