GM2A (179-189) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/4000 (Detection Limit). Western blot: 0.3-1 µg/ml. In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Approx. 24kDa band was observed in Rat Kidney lysates (calculated MW of 21.5kDa according to NP_758838.2 ). Purchase the +ve control! The transfected lysate used to QC this product is available for sale.
Reactivity	Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence (aa 1798-189) from the internal region of the protein sequence according to NP_000396.2.
Specificity	This antibody is expected to recognize isoform 1 (NP_000396.2) only.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	GM2 ganglioside activator
Database Link	Entrez Gene 2760 Human UniProt ID P17900
Background	This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease.
Synonyms	Ganglioside GM2 activator, GM2-AP, Cerebroside sulfate activator protein, Shingolipid activator protein 3
Reference Data

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