TNFRSF1B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, FN, IP, WB
Recommend Dilution	Flow Cytometry: The typical starting working dilution is 1/50. Immunoassays. Immunoprecipitation. Western blot: The typical starting working dilution is 1/50.  Functional Assays: Antibody reactivity with cell bound or soluble TNF-RII is not inhibited by high concentrations of Human TNFa.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	The polyclonal antibody recognizes the extracellular part of the Human Tumor Necrosis Factor Receptor type 2 (TNF-RII) of the membrane-bound as well as the soluble receptor. It cross reacts with Human TNF-RI (problematic in case of TNF-RII knockout studies). Shows minimal cross reactivity with Human TNF-alpha.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	tumor necrosis factor receptor superfamily member 1B
Database Link	Entrez Gene 7133 Human UniProt ID P20333
Background	TNF-RII (~75-80 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. Binding of the inherently trimeric TNF-alpha to TNFR1 and TNFR2 induces receptor trimerization and recruitment of several signaling proteins to the cytoplasmic domains of the receptors. Occupancy of TNFR2 results in direct recruitment of TNF Receptor Associated Factor 2 (TRAF2), which in turn recruits TRAF1.
Synonyms	Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNF-R2
Reference Data

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