UBC3B (UBE2R2) (N-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, EM, IHC
Recommend Dilution	Peptide ELISA: 1/4000 (Detection Limit). Western Blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml. Immunoelectron Microscopy: This product stained chromatoid bodies in Rat spermatids. Immunohistochemistry on Paraffin Sections: 2-4 μg/ml. In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the N Terminus of the protein sequence according to NP_060281.
Specificity	Recognizes UBC3B.
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ubiquitin conjugating enzyme E2 R2
Database Link	Entrez Gene 54926 Human UniProt ID Q712K3
Background	Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
Synonyms	Ubiquitin-conjugating enzyme E2 R2, Ubiquitin-protein ligase R2, Ubiquitin carrier protein R2, E2-CDC34B, CDC34B, UBC3B
Reference Data
Protein Pathways	Ubiquitin mediated proteolysis

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