GFP Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot (1/2500-1/5000).  Immuofluorescence. Immunohistochemistry (1/500-1/1000).
Host	Chicken
Clonality	Polyclonal
Immunogen	Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized.
Specificity	Recognizes Green Fluorescent Protein (GFP). Quality Control: Antibodies were analyzed by Western blot analysis (1/5000) and Immunohistochemistry (1/500) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® as the blocking reagent, and HRP-labeled Goat anti-Chicken antibody (Cat.-No AP31795HR-N) as the detection reagent. Immunohistochemistry used Tetramethyl Rhodamine-Labeled anti-Chicken IgY.
Formulation	Sodium Phosphate-Buffered (10 mM, pH7.2) isotonic (0.9%, w/v) Saline with 50% (v/v) Glycerol as an anti-freezing agent and 0.02% (w/v) sodium azide as an anti-microbial agent. State: Aff - Purified State: Liquid purified IgY fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C in the dark. Avoid repeated freezing and thawing. Centrifuge vial before opening.
Database Link	UniProt ID P42212
Background	Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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