Peripherin Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/5000-1/10000 for Western Blots. 1/2000-1/5000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: The antibody was analyzed by Immunohistochemistry (at a dilution of 1/10000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Rat
Host	Chicken
Clonality	Polyclonal
Immunogen	Full-length recombinant Peripherin protein. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.
Specificity	Recognizes Rat Peripherin. Other species not tested.
Formulation	10mM PBS, pH 7.2 containing 1% BSA as stabilizer and 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Database Link	UniProt ID P21807
Background	Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
Synonyms	PRPH, NEF4, PRPH1
Reference Data

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