Spasmolytic Polypeptide (TFF2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human TFF-2 by Direct ELISA (using 100 μl/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2. Sandwich ELISA: To detect Human TFF-2 by Sandwich ELISA (using 100 μl/well) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human TFF-2 (Cat.-No AP31836PU-N/S) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Human TFF-2. Western Blot:To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure E.coli derived Recombinant Human Trefoil Factor 2
Specificity	Recognizes TFF-2
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C for one year. Following reconstitution the antibody can be stored at 2-8°C for 2 weeks. Avoid repeated freezing and thawing.
Gene Name	trefoil factor 2
Database Link	Entrez Gene 7032 Human UniProt ID Q03403
Background	The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464).
Synonyms	TFF2, Spasmolytic polypeptide, SP, Spasmolysin
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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