Smgc Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF
Recommend Dilution	Peptide ELISA: 1/16000 (Detection limit).  Western blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunocytochemistry: 4 µg/ml. Positive staining of select epithelial cells in the murine Submanibular gland at E18. Data provided by Melinda Larsen, State University of New York, Albany, NY.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide: KLEPKYENPTNGS with a Cysteine residue linker, corresponding to internal sequence amino acids 518-530 of Mouse Muc19 (NP_945121.1).
Specificity	This antibody recognizes Mouse Muc19 /Smgc. Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	submandibular gland protein C
Database Link	Entrez Gene 223809 Mouse UniProt ID Q6JHY2
Background	Detected in terminal tubule cells of the submandibular gland (at protein level). Expressed in submandibular salivary glands of 3-day-old males but not adults. Expression in adult submandibular glands is restricted to females. Isoform 5 is expressed in both 3-day-old and adult sublingual glands.
Synonyms	Submandibular gland protein C, Muc19
Reference Data

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