AP2 alpha (AP2A1) (706-727) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, PEP-ELISA, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.01-0.03 μg/ml. Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Reactivity	Bovine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_055018.2.
Specificity	This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	adaptor related protein complex 2 alpha 1 subunit
Database Link	Entrez Gene 160 Human UniProt ID O95782
Synonyms	CLAPA1, Alpha adaptin A, Alpha-1 adaptin, AP2 complex subunit alpha-1
Reference Data

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