AGRP (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, PEP-ELISA
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western Blot:  Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1 and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry on Frozen Sections: 0.05-0.1 μg/ml.  Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the Human infundibular (arcuate) nucleus and their varicose axons.
Reactivity	Canine, Human, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of Human AGRP (NP_001129.1; NP_015531.1).
Specificity	This antibody is expected to recognise both reported isoforms of AGRP (as represented by NP_001129; NP_015531).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	agouti related neuropeptide
Database Link	Entrez Gene 181 Human UniProt ID O00253
Synonyms	AGRT, ART
Reference Data

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