NCAM2 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Peptide ELISA: 1/4000 (Detection limit). Western blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Frozen Sections of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, Figure 1) and moderate peripheral staining outside the glomeruli (top, Figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in Figure 2. Recommended Concentration: 0.5 µg/ml. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml. Paraffin embedded Human Brain (Cortex).
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of the protein sequence according to NP_004531.2.
Specificity	This antibody recognizes NCAM2
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	neural cell adhesion molecule 2
Database Link	Entrez Gene 4685 Human UniProt ID O15394
Synonyms	NCAM21, N-CAM 2
Reference Data

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