LC3B (MAP1LC3B) pThr12 Rabbit Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	Dot Blot: 1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding Thr12 of Human LC3 (APG8b).
Specificity	Recognizes Phospho-LC3B-Thr12
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein A column, followed by two-step phosphospecific peptide affinity purification
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	microtubule associated protein 1 light chain 3 beta
Database Link	Entrez Gene 81631 Human UniProt ID Q9GZQ8
Background	MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms	MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3
Reference Data

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