IGFBP1 (156-170) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit). Western blot: 1-3 μg/ml. Detects a band of Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1).   An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. Immunohistochemistry on Paraffin Sections: 10µg/ml in paraffin embedded Human Testis.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of Human IGFBP1 (NP_000587.1)
Specificity	This antibody recogniztes Human IGFBP1 (156-170).  Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	insulin like growth factor binding protein 1
Database Link	Entrez Gene 3484 Human UniProt ID P08833
Synonyms	IGF-binding protein 1, IGFBP-1, IBP1, IBP-1, Placental protein 12, PP12
Reference Data

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