INS Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IHC, WB
Recommend Dilution	The immunoconjugate is intended for use in different types of highly sensitive fluorescence immunoassays on appropriately treated cell and tissue substrates. This product is not pre-diluted. Recommended Working Dilutions: 1/10-1/40.
Reactivity	Porcine
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Insulin hormone is isolated from Porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.
Formulation	PBS, pH 7.2 Label: FITC State: Lyophilised purified hyperimmune IgG fraction Preservative: None, as it may interfere with the antibody activity. No foreign proteins added Absorption emission: 492 nm- 515 nm Molar radio: Fluorochrome/IgG ~ 2.7
Reconstitution Method	Restore with 1 ml sterile distilled water
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography
Conjugation	FITC
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P01315
Background	Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.
Synonyms	INS
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data

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