Vimentin (VIM) (N-term) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western blot): 1/2000. Immunohistochemistry on Frozen Sections: 1/100. Immunohistochemistry on Paraffin Sections: 1/50 (after microwave treatment). Cytological Material. Incubation Time: 1 h at RT extended with paraffin sections.
Reactivity	Human, Mouse
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic N-terminus of Human Vimentin (2–17).
Specificity	Specific detection of Vimentin (Mr 57,000 polypeptide) in cells and tissues of mesenchymal origin (e.g. fibroblasts, endothelial cells, smooth muscle cells). Tumors Specifically Detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Formulation	State: Serum State: Liquid Stabilized Antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
Synonyms	VIM
Reference Data

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