beta III Tubulin (TUBB3) Mouse Monoclonal Antibody [Clone ID: TU-20]

Specifications

Product Data
Clone Name	TU-20
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1-2 µg/ml, 90 min (Reducing conditions). Positive Control: Porcine brain lysate. Negative Control: HPB-ALL peripheral blood leukemia cell line. Sample Preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Flow Cytometry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Staining Technique: Standard ABC technique (DAB+). Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT or high temperature citrate buffer antigen retrieval. Positive tissue: Neuronal tissue. Immunocytochemistry. Positive Material: Neuro2a mouse neuroblastoma cell line.
Reactivity	Broad
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide (C) 441-448 coupled to Maleimide-activated Keyhole Limpet Hemocyanin via Cysteine added to the N-terminus of the neuron-specific peptide.
Specificity	This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of Human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. This Monoclonal antibody TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate and Caprylic Acid Precipitation
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	tubulin beta 3 class III
Database Link	UniProt ID Q13509
Background	The beta-III Tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Synonyms	Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4
Reference Data

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