Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: H13]

Specifications

Product Data
Clone Name	H13
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1/10. Incubation Time: 1h at RT. Western Blot. ELISA. Detection of C3 deposits in tissue, cells, micro-organisms and immune complexes. It should be used with Positive Control anti-C3b (H11/H206). Non-activated, absorbed C3 contains the C3a determinant, whereas activated C3 is negative with H 13.
Reactivity	Guinea Pig, Human, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Human complement component C3
Specificity	This Monoclonal antibody Clone H 13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H 13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment; C3b or C are negative with H 13.
Formulation	State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml distilled water
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	complement component 3
Database Link	Entrez Gene 718 Human UniProt ID P01024
Background	The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
Synonyms	CPAMD1, Complement component 3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

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