Factor XII (F12) (light chain) Mouse Monoclonal Antibody [Clone ID: 802]

Specifications

Product Data
Clone Name	802
Applications	ELISA, FN
Recommend Dilution	ELISA, to detect/quantitate Factor XII and in coagulation assays.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human Factor XII
Specificity	Reacts with the light chain of Human Factor XII and at 100nM inhibits 50% of Factor XII activity.
Formulation	PBS, pH 7.4, without preservatives State: Azide Free State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C to -80°C. Avoid repeated freezing and thawing.
Gene Name	coagulation factor XII
Database Link	Entrez Gene 2161 Human UniProt ID P00748
Background	Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
Synonyms	Hageman factor, HAF
Reference Data

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