CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: MEM-188]

Specifications

Product Data
Clone Name	MEM-188
Applications	FC, IHC, IP, WB
Recommend Dilution	Western Blot. Flow Cytometry: 3 µg/ml. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1/50. Pretreatment: Heat retrieval (microwave oven) in citrate buffer standard ABC visualization. Positive Tissue: Positive membrane-specific reaction in adjacent peripheral nerve Staining of paraprostatic autonomic ganglion. Staining technique: Standard ABC technique (DAB+).
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	KG-1 Human acute myelogenous leukaemia cell line
Specificity	The antibody reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with Rhesus Monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
Formulation	PBS, pH 7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	neural cell adhesion molecule 1
Database Link	Entrez Gene 4684 Human UniProt ID P13591
Background	CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Synonyms	NCAM-1, N-CAM-1, NCAM
Reference Data

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