CD41-CD61 Complex Mouse Monoclonal Antibody [Clone ID: P2]

Specifications

Product Data
Clone Name	P2
Applications	ELISA, FC, FN, IHC, IP
Recommend Dilution	ELISA. Immunoprecipitation. Functional Assays.  Fluorescence Microscopy or Flow Cytometry: 2 µg / 5x106 platelets/test. Immunohistochemistry (Only suitable on Frozen Sections or Cell Smears): 1/50-1/100.  Not recommended for use in Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human platelets
Specificity	Reacts with gpIIb in the intact complex with gpIIIa, but not with IIb or IIIa, separately. Inhibits platelet aggregation induced by thrombin, collagen and ADP. The Molecular weight of the recognized antigen is 135kDa/95kDa.
Formulation	PBS buffer State: Azide Free State: Lyophilized purified Ig fraction Stabilizer: 1 mg/ml BSA Preservative: None
Reconstitution Method	Restore with 1 ml distilled water.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term.  The addition of 0.09% (w/v) Sodium Azide is recommended for storage of the reconstituted form for up to one month at 2-8°C. Avoid repeated freezing and thawing.
Background	Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation.
Synonyms	GPIIb-IIIa, ITGA2B, GP2B, ITGAB, Platelet membrane glycoprotein IIb, GPalpha IIb, GPIIb, GP3A, CD41, CD61, Integrin alpha-IIb, Integrin beta-3, ITGB3
Reference Data

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