CLTC Mouse Monoclonal Antibody [Clone ID: CHC5.9]

Specifications

Product Data
Clone Name	CHC5.9
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure.
Reactivity	Amphibian, Bovine, Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Coated vesicles (Clathrin) of Bovine brain.
Specificity	Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.
Formulation	State: Purified State: Lyophilized purified IgM fraction.
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Gel filtration
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Database Link	Entrez Gene 281080 Bovine UniProt ID P49951
Background	Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
Synonyms	CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker
Reference Data

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