Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

Specifications

Product Data
Clone Name	ED1
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to labenl 106 cells in 100 µl. Results may be enhanced using membrane permeabilisation . The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
Reactivity	Bovine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Specificity	This antibody clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of 90-110 KDa, also known as rat CD68 (Dijkstra et al. 1985). The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).
Formulation	PBS Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	Cd68 molecule
Database Link	Entrez Gene 287435 Rat UniProt ID Q4FZY1
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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