Sialoadhesin (SIGLEC1) Mouse Monoclonal Antibody [Clone ID: ED3]

Specifications

Product Data
Clone Name	ED3
Applications	FC, IF, IHC, IP
Recommend Dilution	Immunoprecipitation.  Immunofluorescence. Flow Cytometry: Use 10 µl of 1/500 diluted CD169 antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/50-1/250.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Specificity	This antibody recognises the CD169 cell surface antigen. CD169 is a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	sialic acid binding Ig like lectin 1
Database Link	UniProt ID Q9BZZ2
Background	Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
Synonyms	Sialoadhesin, Siglec-1
Reference Data

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