Adgre1 Rat Monoclonal Antibody [Clone ID: BM8]

Specifications

Product Data
Clone Name	BM8
Applications	FC, IF, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1.0 µg/ml (1/200). Immunohistochemistry on Paraffin Sections: not tested, but work well with the purified antibody using Proteinase K pretreatment. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Cultured Macrophages.
Specificity	This antibody is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS. This clone is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution: Isolated Cells: expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 5 mg/ml BSA Preservative: None
Reconstitution Method	Restore with 0.5 ml distilled water
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	adhesion G protein-coupled receptor E1
Database Link	Entrez Gene 13733 Mouse UniProt ID Q61549
Background	F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.
Synonyms	Emr1, Gpf480
Reference Data

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