Adgre1 Rat Monoclonal Antibody [Clone ID: Cl:A3-1]

Specifications

Product Data
Clone Name	Cl:A3-1
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of neat to 1/10 diluted antibody to stain 10e6 cells in 100 µl. Immunohistology on Frozen and Paraffin Embedded Sections: For paraformaldehyde paraffin tissue, pretreatment by trypsinisation for 20-30 minutes is required. Use 1/50 on cryosections, 1/20 on paraffin sections. Western Blot.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognizes the Murine F4/80 antigen, a 160 kD cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).
Formulation	PBS Label: Biotin State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	adhesion G protein-coupled receptor E1
Database Link	Entrez Gene 13733 Mouse UniProt ID Q61549
Background	F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Synonyms	Emr1, Gpf480
Reference Data

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