Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

Specifications

Product Data
Clone Name	CI:A3-1
Applications	FC, IF
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl. Immunofluorescence.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 myeloma cell line.
Specificity	This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. We recommend the use of BM4008LE for this purpose.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	adhesion G protein-coupled receptor E1
Database Link	Entrez Gene 13733 Mouse UniProt ID Q61549
Background	F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGFTM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Synonyms	Emr1, Gpf480
Reference Data

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