CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

Specifications

Product Data
Clone Name	MEM-148
Applications	FC
Recommend Dilution	Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peripheral blood mononuclear cells
Specificity	The antibody MEM-148 recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit; β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Formulation	Phosphate buffered saline (PBS) solution containing 15mM sodium azide Label: APC State: Liquid purified Ig fraction Label: Conjugated with cross-linked Allophycocyanin under optimum conditions
Purification	Size-exclusion chromatography
Conjugation	APC
Storage Condition	Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms	Integrin beta-2, MFI7
Reference Data

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