CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: X-5]

Specifications

Product Data
Clone Name	X-5
Applications	IHC
Recommend Dilution	Suitable for Immunohistochemistry on frozen sections: 7.5 - 30 µg/ml (1/50-1/200). Suggested positive control: Human tonsil Antigen Distribution on Isolated cells and Tissue sections: Weakly positive on granulocytes. Positive on peritoneal macrophages. Negative on lymphocytes. Cell Lines: HL-60: positive on approx. 50% following 1.25% DMSO treatment increased to 100%. In lymph nodes X-5 is positive on monocytes, macrophages and single cells both intrafollicularly and in surrounding blood vessels. Positive staining has been observed with the basal membrane of blood vessels and on sinus walls. Marginal zone macrophages, medulla and red pulp stain positive in the spleen as do cells in the T-cell region. In the thymus, medulla and trabecular macrophages are positive.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human peripheral blood monocytes
Specificity	This antibody reacts with granulocytes, monocytes, NK- cells. X-5 recognizes an antigen of 155/95kD determined by Immunoprecipitation.
Formulation	PBS, pH 7.2 with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer. Label: Biotin State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	1.5 mg/ml
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 3684 Human UniProt ID P11215
Background	Antibody X-5 recognizes a non-covalently bound alpha heterodimeric glycoprotein. It belongs to the ß-2 integrin supergene family of adhesion. The antigen is a receptor for C3bi, fibrinogen, coagulation factor X. Together with CD18, CD11b forms the MAC-1 molecular complex involved in the adhesion to endothelial cells, and the subsequent extravasation of neutrophils and monocytes.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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