Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: X-6]

Specifications

Product Data
Clone Name	X-6
Applications	FC, IHC
Recommend Dilution	Suitable for Immunohistochemistry on frozen sections (2 µg/ml (1:100) and FACS (use 10 µl stock solution to label 10e6 cells).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human monocytes.
Specificity	Detects human granulocytes, monocytes and platelets. X-6 detects an antigen of 154-180kD by immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes. Positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95% cord blood CD34 cells. Weak reaction on resting neutrophils. Staining increase with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes postive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to decrease in no. of positive cells. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
Formulation	PBS, pH 7.2 containing 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% Thimerosal as a preservative. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 5
Database Link	Entrez Gene 3678 Human UniProt ID P08648
Background	The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.
Synonyms	Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

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