CD46 Mouse Monoclonal Antibody [Clone ID: 13/42]

Specifications

Product Data
Clone Name	13/42
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 5-10 µg/ml of neat antibody to label 1x106 cells. Immunoprecipitation: 5 µg/200 µl extract from 2x106 cells. Immunohistochemistry on Frozen Sections: 1-2 µg/ml (1/200-1/400). Immunohistochemistry on Paraffin Sections: 8 µg/ml (1/50). Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS, Western Blots and Functional Studies.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human U-251 cells. Epitope: Localized in the first two short consensus repeats.
Specificity	The monoclonal antibody 13/42 recognizes Human CD46. The recognized epitope is localized on the first two short consensus repeats. Cross-reacts with African green monkey. Antigen Distribution: The antigen is widely expressed on Human cells except erythrocytes. It can also be found as a soluble protein in serum and saliva. Many Human cell lines express CD 46 e.g. U937, HeLa.
Formulation	PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD46 molecule
Database Link	Entrez Gene 4179 Human UniProt ID P15529
Background	CD46 also known as Membrane Cofactor Protein (MCP), acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.
Synonyms	TLX, MIC10
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature. 1. Wash in PBS. 2. Block endogenous peroxidase. 3. Wash in PBS. 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber. 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber. 6. Wash in PBS. 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber. 8. Wash in PBS. 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS. 11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature. 1. Deparaffinize and rehydrate tissue section. 2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS. 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber. 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber. 8. Wash in PBS. 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber. 10. Wash in PBS. 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS. 13. Counterstain with Mayer's hemalum.
Reference Data

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