gamma Catenin (JUP) Mouse Monoclonal Antibody [Clone ID: PG 5.1]

Specifications

Product Data
Clone Name	PG 5.1
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoblotting. Immunoprecipitation. Immunofluorescence Microscopy: 1/5 (with PBS, pH 7.4). Immunohistochemistry on Frozen Sections. Incubation Time: 1 h at RT. Immunohistochemistry on Paraffin Sections has been described (See reference product citation 1, Brodehl, et al, 2013 ).
Reactivity	Bovine, Chicken, Human, Mouse, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Plakoglobin, "band 5" protein from Bovine snout epidermis
Specificity	PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells); Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).
Formulation	Final solution contains PBS buffer, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	junction plakoglobin
Database Link	Entrez Gene 3728 Human UniProt ID P14923
Background	Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.
Synonyms	Desmoplakin-3, Desmoplakin III, Catenin gamma, JUP, CTNNG, DP3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.