Desmoglein 3 (DSG3) Mouse Monoclonal Antibody [Clone ID: Dsg-G194]

Specifications

Product Data
Clone Name	Dsg-G194
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western Blot). Immunohistochemistry on Frozen Sections: Ready-to-use. Incubation Time: 1 h at RT. Recommendations: For better resolution, preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (See Ref.2).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human polypeptide Desmoglein 3 (C-terminal amino acids 920-940)
Specificity	In Immunoblots of cytoskeletal preparations from Human epidermis the antibody reacts specifically with a 140 kD polypeptide typical for Desmoglein 3. In Immunolocalization studies, the mab shows typical staining of basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus vulgaris patient sera. Cross-reaction with Desmogleins 1 and 2 was not detected.
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	desmoglein 3
Database Link	Entrez Gene 1830 Human UniProt ID P32926
Background	Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.
Synonyms	DSG3, CDHF6, PVA, 130 kDa pemphigus vulgaris antigen
Reference Data

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