Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: VIM 3B4]

Specifications

Product Data
Clone Name	VIM 3B4
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Immunoblotting. Flow Cytometry. Immunofluorescence Microscopy (5-10 µg/ml recommended) Immunohistochemistry (Dilute at least 1/10 with PBS, pH 7.4). Suitable for Frozen and Paraffin-Embedded tissue and Cytological Material. With paraffin-embedded sections, protease pretreatment is required prior to antibody application. Incubation Time: 1 h at RT; extended with paraffin.
Reactivity	Amphibian, Bovine, Canine, Chicken, Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Vimentin (purified from Bovine lens)
Specificity	This antibody is highly specific for the intermediate filament protein Vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (Vimentin) of mesenchymal cells. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of Vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine Vimentin (that could explain for the weak cross-reaction of the antibody with murine Vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using Human Vimentin (Rogers et al., 1995). Clone VIM 3B4 has turned out to be the most avid mab to Vimentin. Tumors Specifically Detected: Sarcoma (including myosarcoma), lymphoma, melanoma. Tested Reactivities on Cultured Cell Lines: RD cells, glioma cells, fibroblasts (SV-80), MDCK.
Formulation	Liquid purified Ig fraction with 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1 (FITC)
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is an intermediate filament protein which is present in all cells of mesenchymal origin. Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms	VIM
Reference Data

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