Cardiotin Mouse Monoclonal Antibody [Clone ID: R2G]

Specifications

Product Data
Clone Name	R2G
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded tissue. Recommended Dilutions: 1/25–1/100 for immunohistochemistry with avidinbiotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/25–1/500 for Immunoblotting applications.
Reactivity	Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Total protein extract of Chicken gizzard.
Specificity	R2G recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. This antibody reacts with Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish tissues.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Background	Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Reference Data

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