beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-01]

CAT#: BM604S

beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, Purified



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CNY 3,140.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name B2M-01
Applications ELISA, FC, IF, IHC, IP, R, WB
Recommend Dilution Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli.
Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.
RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Purified Human beta-2-Microglobulin
Specificity he antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
Negative Species: Mouse, Bovine, Canine (Dog), Rabbit and Chicken.
Formulation PBS, pH~7.4
State: Purified
State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Preservative: 15 mM Sodium Azide
Concentration lot specific
Purification Sequential precipitation method with caprylic acid and ammonium sulphate
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Gene Name beta-2-microglobulin
Background Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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