Col4a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	Suitable for immunostaining of extra or intracellular components in light microscopy. RIA (1:5,000). ELISA >1:200 (OD>500). Immuno-fluorescence analysis (with fluorescein anti-rabbit IgG conjugate): >1:80 on frozen mouse tissues (skin, liver). Immunohistochemistry on paraffin sections (immunoperoxidase technique with the Envision/Dako kit): >1:500 on fixed, paraffin-embeded mouse tissues (skin, liver).
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen Type IV extracted and purified from mouse tumor tissues.
Specificity	Cross-Reactivity (RIA%): Mouse Collagen Type IV 100% Mouse Collagen Types I, III < 0.1% Human Collagen Type IV, V < 0.1% Mouse Fibronectin < 0.1% Mouse Laminin < 4.0%
Formulation	State: Purified State: Lyophilized purified Ig fraction without preservatives.
Reconstitution Method	Reconstitute in 0.5 ml deinoized water.
Concentration	lot specific
Purification	Column chromatography.
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. store the reconstituted antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	collagen, type IV, alpha 1
Database Link	Entrez Gene 12826 Mouse UniProt ID P02463
Background	Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Synonyms	COL4A1
Reference Data

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