Perilipin-1 (PLIN1) (C-term) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/2000 (ECL) Immunohistochemistry on Frozen Sections: 1/100-1/200 for 1 h at RT. Immunohistochemistry on Paraffin Sections: 1/100-1/200 for 1 h at RT. Microwave treatment required (for protocol cf. Straub et al. 2008).
Reactivity	Bovine, Human
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	C-terminus of Perilipin-1 (hCTA/B; aa 507-519; cf. Greenberg et al. 1992, JBC 266, 11341-11346)
Specificity	The antiserum reacts specifically with Perilipins located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. The antiserum does not cross-react with Adipophilin (ADRP) or TIP47 proteins (additional members of the PAT-family).
Formulation	State: Serum State: Liquid Stabilized Antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	perilipin 1
Database Link	Entrez Gene 5346 Human UniProt ID O60240
Background	Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively.
Synonyms	Perilipin, PLIN, PLIN1, PLIN-1, PERI
Reference Data
Protein Families	Druggable Genome
Protein Pathways	PPAR signaling pathway

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