Vimentin (VIM) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunoblotting: 1/2000.  Immunohistochemistry on Frozen Sections: 1/100. Immunohistochemistry on Paraffin Sections: 1/50.  Incubation Time: 1 h at RT, extended with paraffin sections.
Reactivity	Bovine, Chicken, Hamster, Human, Mouse, Xenopus
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Vimentin purified from calf lens
Specificity	Specific detection of Vimentin (Mr 57,000 polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Formulation	State: Serum State: Liquid Stabilized Antiserum Preservative: 0.09% Sodium Azide Stabilizer: 0.5% BSA
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms	VIM
Reference Data

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